Hop cones (Lupuli flos) are rich in biologically active compounds. Among them, 8-prenylnaringenin (8PN) stands out due to its promising anti-inflammatory properties in the treatment of skin diseases. This compound has attracted attention from scientists across disciplines – including chemical engineering, biotechnology, and pharmacology.

The goal of the research project is to develop a method for producing a hop extract enriched with 8PN, which could become the basis for novel therapies, including treatments for atopic dermatitis.

– We aim not only to obtain the extract but also to enhance it by transforming selected compounds found in hops – such as xanthohumol and isoxanthohumol – into 8PN – explains Jan Krzysztoforski, PhD, project leader on behalf of WUT. – This is possible thanks to a combination of chemical and biotechnological transformations, including the use of microorganisms.

What will the research teams focus on?

Researchers from the Faculty of Chemical and Process Engineering at WUT will work on an efficient method of extracting key active compounds from hop cones using supercritical CO₂ extraction under high pressure. They will examine how variables such as temperature, extraction time, and hop pre-treatment methods influence both the yield and composition of the extract.

In the next phase, selected compounds from hops will be biochemically converted into 8PN. The transformations will be carried out using microorganisms and chemical processes in laboratory-scale reactors and bioreactors.

In the third phase, the team will develop mathematical models of the supercritical extraction process. These will allow researchers to simulate different production conditions without the need for repeated experiments. They will employ both AI-based algorithms and advanced 3D simulations, enabling insight into what occurs within the extraction system. The models will support optimization of the process for efficiency and cost-effectiveness.

The second part of the project, conducted by researchers from WUM, will investigate the therapeutic potential of 8PN through laboratory tests on human skin cells, the skin microbiota of volunteers, and animal models of atopic dermatitis. The aim is to confirm the effectiveness of 8PN-enriched hop extract as a basis for new medicinal formulations.

Not just research, but real collaboration

The project is a model of effective interdisciplinary cooperation. On the WUM side, the research is led by Weronika Skowrońska, PhD, from the Faculty of Pharmacy. At WUT, aside from the project leader, a key role is played by Katarzyna Dąbkowska-Susfał, PhD.

– This project is a continuation of my research work at the interface of chemical engineering and biotechnology – says Krzysztoforski, PhD. – It allows for the development of the SCF-LAB and our research team, while also strengthening collaboration with WUM and ETH Zürich, opening the way for further joint initiatives.

From lab to patient

The project aims to develop a novel, green-chemistry-based production method for hop extracts enriched with 8-prenylnaringenin (8PN). By combining experimental studies with advanced modeling, researchers will gain a deeper understanding of the supercritical extraction mechanisms and identify optimal process conditions. Simultaneously, the therapeutic efficacy of the extracts will be assessed in the context of inflammatory skin conditions.

In the long term, this research may lead to the development of innovative medicinal products derived from hops, produced sustainably and aligned with the principles of green chemistry and sustainable development.

– This is science with real potential to impact patients’ lives – and that’s our greatest motivation – the researchers emphasize.

The project "Biotechnological method for obtaining 8-prenylnaringenin for application in the treatment of inflammatory skin diseases" is being carried out in partnership between Warsaw University of Technology and the Medical University of Warsaw, in cooperation with ETH Zürich. It is funded by the National Science Centre (NCN) under the SONATA 20 call (project no. 2024/55/D/ST8/02357), with a total budget of PLN 1,197,430.